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β lactamase inhibitors avibactam  (MedChemExpress)


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    MedChemExpress β lactamase inhibitors avibactam
    β Lactamase Inhibitors Avibactam, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2+lactamase+inhibitors+avibactam/Avibactam/pm41860015-34-21-31
    Average 95 stars, based on 49 article reviews
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    In Vitro:

    Article Title: Unravelling the triad of penicillin-binding proteins, β-lactamase activity, and mRNA dynamics in Pseudomonas aeruginosa AmpC induction.
    Article Snippet: .. Bacterial strains and in vitro susceptibility testing P. aeruginosa PAO1 and its isogenic LMW-PBPs knockouts, PAOΔdacB, PAOΔdacBdacC, and PAOΔdacBdacCpbpG (which our group previously documented as constitutively hyper-expressing AmpC), were utilized in this study.11 MICs for imipenem, doripenem, meropenem, ertapenem, cefepime, ceftazidime, cefoxitin, aztreonam, piperacillin, ticarcillin, carbenicillin, mecillinam, and the β-lactamase inhibitors avibactam, clavulanic acid, relebactam, tazobactam, and sulbactam (all from MedChem Express, Sollentuna, Sweden) were determined by Clinical and Laboratory Standards Institute (CLSI) broth microdilution.23 ..



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    Antibiotic single and combinatorial therapies in a murine subcutaneous abscess model. P. aeruginosa LESB58 was injected subcutaneously and treated one hour later with either vehicle, single antibiotics or an antibiotic combination. Abscess sizes are shown as box and whisker plots (left panel) and counted bacteria recovered from the abscess tissue as CFU/abscess with geometric mean and geometric standard deviation (right panel). Combinatorial treatment of ceftazidime (CAZ; 50 mg/mL) or ceftazidime/avibactam (CZA; 50 mg/mL) with (A) azithromycin (AZM; 6.25 mg/mL), (B) colistin (CST; 1.25 mg/mL), (C) tigecycline (TGC; 2.5 mg/mL), or (D) tobramycin (TOB; 2 mg/mL). A-D. VEH for AZM experiments was 1.56 mg/mL citric acid in 0.9% saline at pH 7-7.2; VEH for all other experiments was 0.9% saline. VEH, CAZ & CZA mice are shared for CST, TGC & TOB graphs. All experiments were done at least three times with 3-5 mice (6-8 week, female Swiss Webster)/group. Statistical analysis was performed using One-way ANOVA, Kruskal Wallace test with Dunn’s correction (two-sided). Limit of detection for CFU counts was 10 2 . Asterisks indicate significant effect of treatment compared to vehicle control (* p < 0.05; ** p < 0.01), and hashes indicate a significant effect of the combination therapy over the sum of the effects of each agent alone ( # p < 0.05; ## p < 0.01).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Using host-mimicking conditions and a murine cutaneous abscess model to identify synergistic antibiotic combinations effective against Pseudomonas aeruginosa

    doi: 10.3389/fcimb.2024.1352339

    Figure Lengend Snippet: Antibiotic single and combinatorial therapies in a murine subcutaneous abscess model. P. aeruginosa LESB58 was injected subcutaneously and treated one hour later with either vehicle, single antibiotics or an antibiotic combination. Abscess sizes are shown as box and whisker plots (left panel) and counted bacteria recovered from the abscess tissue as CFU/abscess with geometric mean and geometric standard deviation (right panel). Combinatorial treatment of ceftazidime (CAZ; 50 mg/mL) or ceftazidime/avibactam (CZA; 50 mg/mL) with (A) azithromycin (AZM; 6.25 mg/mL), (B) colistin (CST; 1.25 mg/mL), (C) tigecycline (TGC; 2.5 mg/mL), or (D) tobramycin (TOB; 2 mg/mL). A-D. VEH for AZM experiments was 1.56 mg/mL citric acid in 0.9% saline at pH 7-7.2; VEH for all other experiments was 0.9% saline. VEH, CAZ & CZA mice are shared for CST, TGC & TOB graphs. All experiments were done at least three times with 3-5 mice (6-8 week, female Swiss Webster)/group. Statistical analysis was performed using One-way ANOVA, Kruskal Wallace test with Dunn’s correction (two-sided). Limit of detection for CFU counts was 10 2 . Asterisks indicate significant effect of treatment compared to vehicle control (* p < 0.05; ** p < 0.01), and hashes indicate a significant effect of the combination therapy over the sum of the effects of each agent alone ( # p < 0.05; ## p < 0.01).

    Article Snippet: The β-lactamase inhibitor avibactam (AVI; MedChem Express) was used alone or in combination with ceftazidime (ceftazidime/avibactam; CZA).

    Techniques: Injection, Whisker Assay, Bacteria, Standard Deviation, Saline, Control